Description
KN-300 Gel Permeation Chromatography (GPC)
Overview
As we have seen, GPC/SEC is a type of LC and so solid stationary and liquid mobile phases are again used. However, the separation mechanism here relies solely on the size of the polymer molecules in solution, rather than any chemical interactions between particles and the stationary phase.
Features：
- High-precision high-pressure constant-flow pump, stable infusion, good repeatability
- Real-time pressure display, high and low pressure alarms and other functions to ensure the safety of the instrument
- Variable wavelength ultraviolet-visible detector, wide wavelength range, high sensitivity
- Touch screen automatic fraction collector, easy to operate and intuitive
- Stainless steel and quartz glass gel purification columns are optional, with good consistency, shortening operation time and improving work efficiency
- Floating self-calibration sealing ring technology and patented technology of pump head self-cleaning function, which can extend the service life of the sealing ring and ensure the durability and high efficiency of the instrument
- Convenient and easy-to-use flow and pressure correction function to ensure the high precision and stability of the instrument
- Adopt two kinds of gradient running program design: linear gradient and step gradient are optional
- Programmable solvent type and compression factor, which can automatically compensate for the complete pre-cleaning function, which is convenient for quick solvent replacement
- Adopts high-precision photodiode design, linear range expansion
- Convenient and unique light intensity gain coefficient self-correction function
- Unique holmium glass cell design, full wavelength scanning, convenient and accurate wavelength correction
- Universal sample cell design, easy to change between micro and constant cell
Configuration:
| No. | Name | Specification | Unit | Qty. |
| 1 | GPC | High pressure infusion pump 1set
Variable wavelength ultraviolet detector 1set Large volume manual injection valve 1pcs Gel purification column (filled with imported gel filler to meet the pre-treatment of food or environmental samples) 1pcs Chromatography workstation 1set Manual fraction collector 1pcs |
set | 1 |
Technical parameters
| High pressure constant flow pump | |
| Measuring range | 0.001-10ml/min (Step size setting : 0.001mL/min) |
| Max working pressure | 42.0MPa Upper and lower limits can be set, automatic alarm |
| Flow accuracy | ±1.0％ or 2uL/min（0.001－10mL/min） |
| Flow precision | 0.25% |
| Gradient | Double |
| Gradient method | Gradient elution internal software can be self-controlled, and can be controlled by PC and pump |
| Rated voltage | AC 110V/220V，50Hz/60Hz |
| Dimension | 400mm×300mm×180mm |
| Detector | |
| Lamp source | Deuterium Lamp / Tungsten Lamp |
| Wavelength range | 190-700nm, increment : 1nm |
| Bandwidth | <6m |
| Wavelength accuracy | ±1nm |
| Wavelength repeatability | 0.5nm |
| 0.0005AU/mV-4AU/mV | |
| Noise | ±5×10-5AU |
| Drift | 2×10-4AU（P-P） |
| Sample cell | Analysis, Semi-prepared is optional |
Application
Food：
America：AOAC 984.21 、FDA2905A
Europe：EN12393、EN1528、S-19、S35L00.00-34
China：GBT 5009.19-2008，GBT 5009.146-2008，GBT 5009.162-2008，GBT 5009.207-2008，GBT 5009.218-2008，GBT 19650-2006，SN/T2430-2010 ，SN/T 2457-2010 ，SN/T 2459-2010 ，SN/T 2431-2010 ，GB/T20772-2006
Medicine：
2015 Pharmacopoeia 2341: Determination of residues of some pesticides in medicinal materials, decoction pieces and preparations, determination methods of organochlorine and organophosphorus pesticide residues
Environment：
Polycyclic aromatic hydrocarbons, polychlorinated biphenyls, mycotoxins, pigment separation
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